APPLICATIONS OF TECHNOLOGY:
- Mammalian cell transfection
- Bacterial transformation
- Gene editing (CRISPR/Cas9)
- Enables 100x fewer reagents to be consumed per experiment
- Demonstrates up to 380x improvement in transformation efficiency and drastically higher throughput when compared to conventional electroporation platforms
- Capable of performing a parametric study of voltage waveforms to maximize strain specific transformation efficiency
- Electroporation is an important and commonly used process in mammalian cell transfection, bacterial transformation, RNAi, and gene editing (e.g., CRISPR/Cas9). For instance, electroporation works very efficiently in a myriad of cell lines and primary cells that are difficult to transfect by conventional chemical-based transfection methods. However, the current electroporation platforms are costly (i.e. require many expensive biological reagents), time-intensive, and exhibit lower throughput and transformation efficiency.
Researchers at Sandia National Labs and JBEI have developed a high throughput, scalable system for electroporation of biological cells.
The versatile two-part system comprises an electroporation reaction array that interfaces with a control unit, providing electrical pulse and temperature controls. The control unit enables electroporation, cell recovery, and cell outgrowth to be performed in one disposable device in an automated fashion. Ultimately, it increases transformation efficiency by increasing cell viability. Also, the electrode configuration allows for processing of smaller sample volumes, reducing the cost per experiment and the consumption of reagents by several orders of magnitude compared to cuvette-based electroporation devices. These advantages are made possible through hydrophilic treatment of the electrodes. Such a configuration is optimally used for reduced reaction volumes ranging from 0.1 nL to 10 μL.
By decreasing the amounts of experimental steps and consumable waste, this drastically simplified process flow enhances the overall process efficiency. It has applications in mammalian cell transfection, bacterial transformation, RNAi, and gene editing.
DEVELOPMENT STAGE: Proven principle
FOR MORE INFORMATION:
STATUS: Patent pending.
OPPORTUNITIES: Available for licensing or collaborative research.
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