APPLICATIONS Biofuel production Glycobiology Drug discovery Enzyme bioengineering ADVANTAGES Allows native substrates to screen for specific enzymatic activities Small sample volume, high throughput Potential to aid explanation of enzyme catalytic mechanism ABSTRACT Creating optimal enzymes to improve the efficiency of converting biomass into sugars … [Read more...] about Rapid Discovery and Optimization of Enzyme Solutions Using Tagged Biomass and Mass Spectrometry EJIB-3262
Synthetic Biology Tools and Software
NIMS-based Multiplexed Enzymatic Assays EJIB-2926
APPLICATIONS OF TECHNOLOGY: Biofuel development Industrial enzyme manufacturing ADVANTAGES: Enables evaluation of several enzymatic reactions in parallel Can be used directly with crude environmental samples Potential for rapid parallel analysis with low sample volumes ABSTRACT: Trent Northen and colleagues at Berkeley Lab and JBEI have developed a rapid … [Read more...] about NIMS-based Multiplexed Enzymatic Assays EJIB-2926
Production of Bacterial Microcompartments for Synthetic Biology Applications IB- 2013-014
APPLICATIONS OF TECHNOLOGY: Biotechnology Carbon capture and sequestration Biofuels production Drug delivery Specialty chemical manufacturing Vaccine development ADVANTAGES: Small, uniform geometries Long lasting (> 2 months) Semipermeable membrane walls Allows engineered pore selectivity Does not rely on natural operon sequences Well … [Read more...] about Production of Bacterial Microcompartments for Synthetic Biology Applications IB- 2013-014
Irreversible, Low Load Genetic Switches EJIB-2593
APPLICATIONS OF TECHNOLOGY: Induction/suppression of gene expression Production of genomic knock-outs and knock-ins Growing genetically engineered crops for biofuel feedstock Targeted gene therapy Containment systems for environmental cleanup Research by developmental and systems biologists, biological engineers and synthetic biologists ADVANTAGES: Handles … [Read more...] about Irreversible, Low Load Genetic Switches EJIB-2593
Directed Evolution of Microbe Producing Biofuels Using In Vivo Transcription Factor Based Biosensors EJIB-2710
APPLICATIONS OF TECHNOLOGY: Improved production of C2-C6 linear alcohols and aldehydes Alcohol-based induction system for gene regulation and protein overexpression ADVANTAGES: High throughput screening and selection Improved sensitivity for measuring enzyme activity and small molecule production Broad applicability Single cell resolution … [Read more...] about Directed Evolution of Microbe Producing Biofuels Using In Vivo Transcription Factor Based Biosensors EJIB-2710