- Nanopore sequencing (i.e. significantly more accurate base caller for nanopore data)
- Streaming base calling
- Base calling from information-rich raw signal
- Nanopore applications require base calling. Current nanopore base calling software applications employ the Viterbi algorithm, which requires the whole sequence to employ the complete base calling procedure and thus precludes a natural streaming base calling procedure.
Researchers at Berkeley Lab developed the basecRAWller software, the first base caller for nanopore data that calls bases directly from raw data.
In comparison to other sequencing technologies, the basecRAWller algorithm has the powerful ability to perform truly streaming base calling as signal is received from the sequencer. The other major advantage is the prediction of bases from raw signal, which contains much richer information than the segmented chunks that current algorithms employ.
As a result, basecRAWller leads to base calls of much greater accuracy.
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OPPORTUNITIES: Available for licensing (free for academia, fee for commercial use)