APPLICATIONS: Molecular biology assays (TUNEL assay) Enzymatic de novo DNA synthesis with free dTNPs and polymerase-dNTP conjugates at elevated temperatures BENEFITS: Enhanced thermostability Increases the elongation rate for de novo DNA synthesis BACKGROUND: Terminal deoxynucleotidyl transferase (TdT) is used in several molecular biology methods. A … [Read more...] about Terminal deoxynucleotidyl transferase variants with enhanced thermostability 2019-015
Biofuels
A Novel D-Xylose Isomerase for Efficient Lignocellulosic Hydrolysate Fermentation 2019-132
APPLICATIONS OF TECHNOLOGY: Genetic modification of Saccharomyces cerevisiae for pentose fermentation and glucose-fructose isomerization Improved lignocellulose hydrolysate fermentation BENEFITS: Relative to current enzymes, this novel xylose isomerase shows: More than 2.5 times higher specific activity Almost 40% lower Km for D-xylose Higher activity … [Read more...] about A Novel D-Xylose Isomerase for Efficient Lignocellulosic Hydrolysate Fermentation 2019-132
Efficient Production of Oxidized Terpenes Via Enzyme Fusion Strategy 2019-108
APPLICATIONS OF TECHNOLOGY: Production of oxidized terpenes and of terpene-based bioproducts (e.g. fuel additives, fragrances, insecticides, pharmaceuticals) BENEFITS: Enables more efficient production of functionalized terpenes BACKGROUND: The functionalization of terpene hydrocarbons using cytochrome P450 enzymes could derive many useful compounds that … [Read more...] about Efficient Production of Oxidized Terpenes Via Enzyme Fusion Strategy 2019-108
Improving High Value Carotenoid Astaxanthin Production 2017-033
APPLICATIONS OF TECHNOLOGY: Human health and nutrition Biofuel feedstocks Food production ADVANTAGES: More economically viable means to produce microalgae Identifies specific genes required for astaxanthin antioxidant synthesis ABSTRACT: Researchers led by Melissa Roth of Howard Hughes Medical Institute and Krishna Niyogi of the Berkeley Lab have … [Read more...] about Improving High Value Carotenoid Astaxanthin Production 2017-033
Specific Reduction of Various Isoprenoid Precursors and Isoprenoids by Geranylgeranyl Reductases 2018-088
APPLICATIONS OF TECHNOLOGY: Bio-derived platform chemicals for use in Pharmaceuticals Cosmetics and fragrances Fuels ADVANTAGES: Lower cost, bio-based pathway to high value chemicals Reduction of intermediates within the terpene biosynthesis pathway Production of higher quantity of farnesyl acetate ABSTRACT: Researchers at Berkeley Lab’s Joint … [Read more...] about Specific Reduction of Various Isoprenoid Precursors and Isoprenoids by Geranylgeranyl Reductases 2018-088