APPLICATIONS OF TECHNOLOGY: DNA Assembly & Cloning Biological Research Sequencing Protein Expression ADVANTAGES: One pot cloning, regardless of internal restriction sites Assembly using various restriction enzymes BACKGROUND: Golden Gate assembly is the preferred DNA assembly technique for repetitive DNA elements and secondary structure, as … [Read more...] about One-Pot Golden Gate Cloning via Methylation and Chemically Modified Assisted Tailorable Ends (McMaster) 2023-035
Biotechnology
Genetically Modified Host Cells for Improved Polyketide Production 2022-152
APPLICATIONS OF TECHNOLOGY: High-value products using methylmalonyl-CoA as substrate: Erythromycin (antibiotic) and spinosyn (insecticide) Germicidin, which has significant inhibitory activity on hexokinase which is expressed at a high level in cancer cells Novel recyclable materials Flavors and fragrances Commodity or specialty chemicals ADVANTAGES: … [Read more...] about Genetically Modified Host Cells for Improved Polyketide Production 2022-152
Metabolic engineering of Issatchenkia orientalis for citramalate production used in PMMA Acrylic Glass 2023-056
APPLICATIONS OF TECHNOLOGY: Production of citramalate, a precursor for polymethyl methacrylate (PMMA) polymers. PMMA is used in electronic device screens, automotive plastics, glazing, building piping, and insulation ADVANTAGES: Increases the achievable carbon yields of select organic acids Consumes less energy and generates less toxic waste than current … [Read more...] about Metabolic engineering of Issatchenkia orientalis for citramalate production used in PMMA Acrylic Glass 2023-056
Expression Platform for Soluble Hydrogenase Production via Escherichia coli 2022-146
APPLICATIONS OF TECHNOLOGY: Renewable hydrocarbon production for biofuels such as biodiesel and ethanol E. coli that can use or produce H2 with its cellular processes, meaning H2 can now be used as a feedstock for biosynthesis. ADVANTAGES: Simplified and efficient expression system compared to traditional methods that use engineered Cupriavidus necator H16 … [Read more...] about Expression Platform for Soluble Hydrogenase Production via Escherichia coli 2022-146
Chelating Ligands for Enhanced Radiolabeling and Therapeutic Potential 2023-119
APPLICATIONS OF TECHNOLOGY: Development of radiopharmaceuticals for cancer diagnosis and treatment Development of antibodies labeled with radionuclides BENEFITS: Simplified synthesis compared to other structures Faster radiolabeling at room temperature Formation of highly stable radiometal complexes Higher synthetic yields BACKGROUND: Thorium-227 … [Read more...] about Chelating Ligands for Enhanced Radiolabeling and Therapeutic Potential 2023-119