APPLICATIONS OF TECHNOLOGY: DNA synthesis ABSTRACT: Oligonucleotides are almost exclusively synthesized using the nucleoside phosphoramidite method, even though it is limited to the direct synthesis of ~200 mers and produces hazardous waste. Researchers at the Joint BioEnergy Institute (JBEI) have developed an oligonucleotide synthesis strategy that uses the … [Read more...] about DNA Synthesis Using Tethered dNTPs 2018-103
Synthetic Biology Tools and Software
DNA Construction by Single-molecule Assembly of Capped DNA Units 2013-188
APPLICATIONS OF TECHNOLOGY: Bioengineering ADVANTAGES: Potentially speed DNA synthesis while reducing costs ABSTRACT: Researchers at the Joint BioEnergy Institute (JBEI) have developed a technology for single-molecule construction of DNA. The invention provides for a method comprising: a) providing a reaction chamber comprising a solid support bound to a … [Read more...] about DNA Construction by Single-molecule Assembly of Capped DNA Units 2013-188
Parallel Droplet Manipulation and Digital Microfluidic Droplet Generation, Transport, Splitting and Merging 2017-053
APPLICATIONS OF TECHNOLOGY: Improved digital microfluidic (DMF) technologies to enable chip-based synthetic biology and biological assays Automation of droplet manipulation ADVANTAGES: Facilitates manufacturing and operation of chips for synthetic biology and biological assays Automates aqueous drop generation and manipulation Reduces production … [Read more...] about Parallel Droplet Manipulation and Digital Microfluidic Droplet Generation, Transport, Splitting and Merging 2017-053
ANIcalculator (Average Nucleotide Identity Calculator) 2014-127
APPLICATIONS OF TECHNOLOGY: Genome sequencing ABSTRACT: Increased sequencing of microbial genomes has revealed that prevailing prokaryotic species assignments can be inconsistent with whole genome information for a significant number of species. The long-standing need for a systematic and scalable species assignment technique can be met by the genome-wide Average … [Read more...] about ANIcalculator (Average Nucleotide Identity Calculator) 2014-127
Thermostable RNA-Guided Endonucleases (GEOCAS9) 2017-117
APPLICATIONS OF TECHNOLOGY: Genome editing Genetic engineering Gene therapy Research tools (e.g., high-throughput screening of gene functions in cell lines and in vivo) Creation of transgenic animal models ADVANTAGES: Functions under different conditions than current CRISPR-Cas9 proteins (e.g, thermostable and enzymatically active in a wide temperature … [Read more...] about Thermostable RNA-Guided Endonucleases (GEOCAS9) 2017-117