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Intellectual Property Office

DIVA: DNA Design, Implementation, and Validation Automation Software 2021-102

APPLICATIONS:

  • DNA design, DNA construction, synthetic biology, engineering biology, efficiency metric tracking

BENEFITS:

  • Provides a collaborative web-based graphical user interface supporting and tracking DNA design and construction. Interfaces with other related enabling software to offer additional functionality and reduce friction for the user. 

BACKGROUND:

  • DIVA is a new graphical user interface for the j5 DNA assembly design software. It leverages other software tools including ICE (Inventory of Composable Elements) to source and deposit DNA sequences, OpenVectorEditor to visualize DNA sequences, BOOST for protein sequence back translation (and other functionality), and BLiSS for biosecurity screening. 

TECHNOLOGY OVERVIEW:

Researchers at Berkeley and Sandia National Labs have designed a web-based software platform known as DIVA (Design, Implementation, and Validation Automation), which improves the operational efficiency of companies and research institutions working on DNA design and construction. 

DIVA enables users to collaboratively and visually design DNA constructs and submit the designs to internal resources or outside vendors for construction at scale. Users design DNA with a web-based graphical user interface, submit their designs to a central queue, and after internal or external review, receive their sequence-verified clonal constructs.  Users can track the construction and subsequent sequence validation processes using DIVA.  Completely independent DNA construction tasks can be aggregated into the same multi-well plates and pursued in parallel because the DIVA DNA construction methods are sequence-agnostic and standardized, i.e. they use the same enzymatic master mixes and reaction conditions. 

Since DIVA captures in a central database all DNA construction success and failure rates, including at which stage a given clonal construction procedure failed, the data can be leveraged to refine and track the efficiency of the DNA assembly design process.

FOR MORE INFORMATION:

https://agilebiofoundry.org/capabilities/design/diva-biocad/

Licensing Agreements: DIVA

DEVELOPERS:

  • Hector Plahar
  • Lisa Simirenko
  • Manjiri Tapaswi
  • Steve Lane (Sandia National Laboratories)

STATUS: Copyrighted.

OPPORTUNITIES: Available for licensing (free for academia, fee for commercial use).