APPLICATIONS OF TECHNOLOGY:
- Production of sustainable blue dyes and colorings from renewable lignin carbon streams
ADVANTAGES:
- Reached 77% of maximum theoretical yield of indigoidine from para-coumarate and 7.3 g/L.
- Does not require that cells enter stationary phase before product can accumulate
BACKGROUND:
Production of synthetic dyes in the textile industry has led to massive amounts of pollution and energy consumption. Indigoidine, which is a natural blue dye commonly made from engineered microbes such as E. coli, can serve as an alternative. However, current methods of biological production suffer from low titer, rate, and yield (TRY). Therefore, there is a need for engineered bacteria strains that improve Indigoidine production to achieve commercial production.
TECHNOLOGY OVERVIEW:
Researchers at Berkeley Lab have engineered a strain of Pseudomonas putida KT2440 for growth-coupled bioconversion of para-coumarate to indigoidine. Using adaptive lab evolution and multimodal approaches, a small number of genes were identified for manipulation in P. putida KT2440.
The strain produced 7.3 g/L of indigoidine, which is 77% of the maximum theoretical yield. This is the highest known yield of this compound in the published literature.
DEVELOPMENT STAGE:
Proven Principle
PRINCIPAL INVESTIGATORS:
- Thomas Eng
- Deepanwita Banerjee
- Aindrila Mukhopadhyay
FOR MORE INFORMATION:
STATUS:
Patent Pending
SEE THESE OTHER BERKELEY LAB TECHNOLOGIES IN THIS FIELD:
A CRISPR-Based and Metabolic Modeling Guided Strategy to Maximizing Indigoidine Production 2019-145
High Titer Production of Indigoidine in Rhodosporidium toruloides 2018-129
Evolved Pseudomonas putida KT2440 with Enhanced Xylose and Galactose Utilization 2021-054