High Throughput Characterization of Bacterial Promoters From Their Host Environments 2022-081
This invention, dubbed “PiSeq,” characterizes bacterial promoters and can enable identification of biosensors for chemicals and physical conditions associated with their host’s physiology and environment. Inventors: Tomoya Honda, Yasuo Yoshikuni. Contact: Jeremy Greeter, jgreeter@lbl.gov
High-Throughput Protein Pull-Down for Mapping Transcription Factors, “Biotin DAP-seq” 2021-071
This invention, dubbed “Biotin DAP-seq,” is a clone-free, high-throughput technique for discovering transcription factor (TF) binding sites. This invention would significantly reduce the upfront investment required to clone and express the tagged TFs used in in vitro assays. Inventors: Leo Baumgart, Ronan O’Malley. Contact: Jeremy Greeter, jgreeter@lbl.gov
Dub-seq: Double Barcoded Shotgun Expression Library Sequencing 2015-160
This invention uses dual random barcoded shotgun expression libraries on replicable plasmids and increases the throughput of functional screens by using barcode sequencing (BarSeq) to assess the microbial fitness. Inventors: Vivek Mutalik, Adam Arkin, Adam Deutschbauer, Pavel Novichkov. Contact: Jeremy Greeter, jgreeter@lbl.gov
ARGO: Peptide Barcoding for Genomic Discovery 2015-123
This invention enables combinatorial experimental designs, allowing for multiplexing and ultra high-throughput via mass spectrometry to read out the identity, quantity, and activity of gene products. Inventors: Markus Raad, Curt Fischer, Trent Northen. Contact: Jeremy Greeter, jgreeter@lbl.gov
Lanthanide-Chelator Barcode for Combinatorial Screening Applications 2016-121
This invention can enable faster and more efficient generation of combinatorial conditions, eliminating a critical bottleneck in combinatorial screening which makes it ideal for testing a high diversity of conditions for drug discovery and optimization, biomanufacturing, and diagnostic applications. Inventors: Kai Deng, Todd Duncombe, Trent Northen, Anup Singh. Contact: Robin Johnston, RJohnston@lbl.gov