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Intellectual Property Office

SynTrack: DNA Assembly Workflow Management  2017-023

APPLICATIONS OF TECHNOLOGY:

  • Management and tracking of DNA synthesis and assembly operations
  • Integration of quality control outcomes and the status of construct deliverables for external users

BENEFITS:

  • System with a host of functions to track the DNA build process:
    • Generating Echo pooling instructions based on plate maps
    • Bulk updating of colony or PCR amplification information, fusion PCR, and chewback results
    • Updating with quality assurance and quality control outcomes with .csv and .xlsx template files
    • Re-work assembly workflow enabled before and after sequencing validation

TECHNOLOGY OVERVIEW:

Researchers at Berkeley Lab have developed SynTrack, a biological computer-aided manufacturing (bioCAM) platform that manages the complexity of synthetic biology workflows and tracks the production of synthetic DNA constructs to maximize efficiency. 

SynTrack implements integrated with laboratory automation systems by enabling task-based workflows to offer complete DNA construct management, assembly pipeline control, plate and well content capturing. Functions include:

  • Data Management of the hierarchical DNA assembly of constructs.
  • Tracking the usage of the various parts and transitory DNA fragments.
  • Streamlining DNA construct build processes via workflow template to improve turnaround time. 
  • Flexible handling the various final DNA constructs from different process tasks.
  • Generating pipetting instruction to leverage liquid handling robotic platforms.
  • Genvering vendor DNA fragment order and sample receiving processes.
  • Management of well plate layouts and associated quantity data.
  • Bulk updating of the incorporated QA/QC results with .csv & .xlsx files.
  • Permitting re-work assembly workflow with an earlier task reactivated and early terminating a workflow through shunt operations.
  • Combining multiple batch experiments under Assembly prep tools.
  • Secure, tabular-formatted web interfaces to observe and update the status of DNA constructs with the timestamp.

FOR MORE INFORMATION:

https://www.osti.gov/biblio/1335644-syntrack-dna-assembly-workflow-management-syntrack-v2

DEVELOPERS:

STATUS: Copyrighted

OPPORTUNITIES: Available for licensing (free for academia, fee for commercial use)